Quantification with ELISA
ELISA methods have been applied for protein quantification from biological matrixes over decades. When an antibody for the target protein is available, quantification using ELISA is sensitive and specific alternative, requiring relatively low sample volumes.
We offer protein quantification using commercially available ELISA kits, assays provided by the customer or we can develop ELISA methods when there is an antibody or antibody pair available for the target protein. The assays can be customised, optimised and verified according to your requirements.
ELISA is an efficient technique and the results correlate with the LC/MS/MS approach. Optimal protein quantification would use two independent analysis (i.e. ELISA + LC/MS/MS).