Cytotoxicity ScreeningIn vitro Toxicology
• Cell membrane integrity (LDH release)
• Cell viability (ATP content)
• HepaRG cells or other preferable cell type
Cytotoxicity (i.e. being toxic to cells) is almost by default an unwanted characteristic in drug discovery and development since cytotoxic compounds could have serious adverse effects. Fortunately, cytotoxicity can be studied by several in vitro methods based on measuring cell viability or cell death.
In our cell membrane integrity assay, cell death is assessed by measuring LDH release to cell culture medium, dead cell specific protease activity or using DNA-binding dye. Cell viability can be assessed in parallel to cell death by measuring the amount of ATP as dead or dying cells have diminished ATP levels. All our cytotoxicity assays utilise fluorescence/luminescence analysis and deliver % cytotoxic / viable cells compared to control toxicant / vehicle. Comprehensive studies also deliver EC50 values.