• In vitro hemolysis
A form of hematologic toxicity can be detected with in vitro hemolysis assay. In hemolysis, blood erythrocytes disrupt leading to hemoglobin release to plasma. According to FDA, it is of importance to detect the hemolytic potential of formulation excipients used for i.v. administration. Drugs and their metabolites may cause direct or immunological, delayed hemolysis. This assay detects the direct hemolysis as %, and also the rate of hemolysis can be determined.