Our services for Permeability and Transporters research
In vitro permeability assays with Caco-2 or MDCK(-MDR1/BCRP) cell lines are used for evaluating both passive and active transport of drugs to blood circulation across the intestinal membranes. The Caco-2 is most often used for evaluating overall permeability and involvement of efflux-transporters or active uptake in a bidirectional experiment.
The MDCK-cells are a convenient tool for assessment of efflux transporters MDR1 and BCRP (and their inhibition) as cell lines overexpressing these transporters are available.
Several assays are also available for studies with individual uptake transporters to investigate whether the new drug candidate is a substrate (potential DDI victim), or an inhibitor (potential DDI perpetrator) towards drug transporters.